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eb3 dynamics  (Nikon)


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    Structured Review

    Nikon eb3 dynamics
    Microtubule defects are not detected in MZ camsap2a mutant embryos. (A) Alpha-tubulin antibody staining in wild-type, MZ camsap2a uot19 (uot19) and MZ camsap2a uot20 (uot20) embryos at 7 hpf (60-70% epiboly). Arrowheads indicate unlabeled external yolk syncytial nuclei surrounded by microtubules. (B-D) Stills from confocal time-lapse movies of EMTB-3XGFP-labeled microtubules in wild-type (B), uot19 (C) and uot20 (D). Arrowheads indicate the yolk-blastoderm boundary. Imaging started at 6 hpf (shield). Time stamp indictates min:s. Scale bars: 50 µm. (E) <t>EB3-GFP</t> in the e-YSL of wild-type embryos at 7 hpf and time-matched mutant embryos. Arrowheads indicate horizontal <t>EB3-GFP.</t> Scale bars: 30 µm. (F) Quantification of e-YSL EB3-GFP speed in wild-type ( n =6) and mutant embryos (uot19, n =7; uot20, n =13). Data are mean±s.e.m. P =0.8792 (left), P =0.6702 (right) (Welch's t -test). ns, not significant; WT, wild type.
    Eb3 Dynamics, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 10098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eb3 dynamics/product/Nikon
    Average 99 stars, based on 10098 article reviews
    eb3 dynamics - by Bioz Stars, 2026-05
    99/100 stars

    Images

    1) Product Images from "Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epiboly"

    Article Title: Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epiboly

    Journal: Development (Cambridge, England)

    doi: 10.1242/dev.204843

    Microtubule defects are not detected in MZ camsap2a mutant embryos. (A) Alpha-tubulin antibody staining in wild-type, MZ camsap2a uot19 (uot19) and MZ camsap2a uot20 (uot20) embryos at 7 hpf (60-70% epiboly). Arrowheads indicate unlabeled external yolk syncytial nuclei surrounded by microtubules. (B-D) Stills from confocal time-lapse movies of EMTB-3XGFP-labeled microtubules in wild-type (B), uot19 (C) and uot20 (D). Arrowheads indicate the yolk-blastoderm boundary. Imaging started at 6 hpf (shield). Time stamp indictates min:s. Scale bars: 50 µm. (E) EB3-GFP in the e-YSL of wild-type embryos at 7 hpf and time-matched mutant embryos. Arrowheads indicate horizontal EB3-GFP. Scale bars: 30 µm. (F) Quantification of e-YSL EB3-GFP speed in wild-type ( n =6) and mutant embryos (uot19, n =7; uot20, n =13). Data are mean±s.e.m. P =0.8792 (left), P =0.6702 (right) (Welch's t -test). ns, not significant; WT, wild type.
    Figure Legend Snippet: Microtubule defects are not detected in MZ camsap2a mutant embryos. (A) Alpha-tubulin antibody staining in wild-type, MZ camsap2a uot19 (uot19) and MZ camsap2a uot20 (uot20) embryos at 7 hpf (60-70% epiboly). Arrowheads indicate unlabeled external yolk syncytial nuclei surrounded by microtubules. (B-D) Stills from confocal time-lapse movies of EMTB-3XGFP-labeled microtubules in wild-type (B), uot19 (C) and uot20 (D). Arrowheads indicate the yolk-blastoderm boundary. Imaging started at 6 hpf (shield). Time stamp indictates min:s. Scale bars: 50 µm. (E) EB3-GFP in the e-YSL of wild-type embryos at 7 hpf and time-matched mutant embryos. Arrowheads indicate horizontal EB3-GFP. Scale bars: 30 µm. (F) Quantification of e-YSL EB3-GFP speed in wild-type ( n =6) and mutant embryos (uot19, n =7; uot20, n =13). Data are mean±s.e.m. P =0.8792 (left), P =0.6702 (right) (Welch's t -test). ns, not significant; WT, wild type.

    Techniques Used: Mutagenesis, Staining, Labeling, Imaging



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    eb3 dynamics  (Nikon)
    99
    Nikon eb3 dynamics
    Microtubule defects are not detected in MZ camsap2a mutant embryos. (A) Alpha-tubulin antibody staining in wild-type, MZ camsap2a uot19 (uot19) and MZ camsap2a uot20 (uot20) embryos at 7 hpf (60-70% epiboly). Arrowheads indicate unlabeled external yolk syncytial nuclei surrounded by microtubules. (B-D) Stills from confocal time-lapse movies of EMTB-3XGFP-labeled microtubules in wild-type (B), uot19 (C) and uot20 (D). Arrowheads indicate the yolk-blastoderm boundary. Imaging started at 6 hpf (shield). Time stamp indictates min:s. Scale bars: 50 µm. (E) <t>EB3-GFP</t> in the e-YSL of wild-type embryos at 7 hpf and time-matched mutant embryos. Arrowheads indicate horizontal <t>EB3-GFP.</t> Scale bars: 30 µm. (F) Quantification of e-YSL EB3-GFP speed in wild-type ( n =6) and mutant embryos (uot19, n =7; uot20, n =13). Data are mean±s.e.m. P =0.8792 (left), P =0.6702 (right) (Welch's t -test). ns, not significant; WT, wild type.
    Eb3 Dynamics, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eb3 dynamics/product/Nikon
    Average 99 stars, based on 1 article reviews
    eb3 dynamics - by Bioz Stars, 2026-05
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Microtubule defects are not detected in MZ camsap2a mutant embryos. (A) Alpha-tubulin antibody staining in wild-type, MZ camsap2a uot19 (uot19) and MZ camsap2a uot20 (uot20) embryos at 7 hpf (60-70% epiboly). Arrowheads indicate unlabeled external yolk syncytial nuclei surrounded by microtubules. (B-D) Stills from confocal time-lapse movies of EMTB-3XGFP-labeled microtubules in wild-type (B), uot19 (C) and uot20 (D). Arrowheads indicate the yolk-blastoderm boundary. Imaging started at 6 hpf (shield). Time stamp indictates min:s. Scale bars: 50 µm. (E) EB3-GFP in the e-YSL of wild-type embryos at 7 hpf and time-matched mutant embryos. Arrowheads indicate horizontal EB3-GFP. Scale bars: 30 µm. (F) Quantification of e-YSL EB3-GFP speed in wild-type ( n =6) and mutant embryos (uot19, n =7; uot20, n =13). Data are mean±s.e.m. P =0.8792 (left), P =0.6702 (right) (Welch's t -test). ns, not significant; WT, wild type.

    Journal: Development (Cambridge, England)

    Article Title: Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epiboly

    doi: 10.1242/dev.204843

    Figure Lengend Snippet: Microtubule defects are not detected in MZ camsap2a mutant embryos. (A) Alpha-tubulin antibody staining in wild-type, MZ camsap2a uot19 (uot19) and MZ camsap2a uot20 (uot20) embryos at 7 hpf (60-70% epiboly). Arrowheads indicate unlabeled external yolk syncytial nuclei surrounded by microtubules. (B-D) Stills from confocal time-lapse movies of EMTB-3XGFP-labeled microtubules in wild-type (B), uot19 (C) and uot20 (D). Arrowheads indicate the yolk-blastoderm boundary. Imaging started at 6 hpf (shield). Time stamp indictates min:s. Scale bars: 50 µm. (E) EB3-GFP in the e-YSL of wild-type embryos at 7 hpf and time-matched mutant embryos. Arrowheads indicate horizontal EB3-GFP. Scale bars: 30 µm. (F) Quantification of e-YSL EB3-GFP speed in wild-type ( n =6) and mutant embryos (uot19, n =7; uot20, n =13). Data are mean±s.e.m. P =0.8792 (left), P =0.6702 (right) (Welch's t -test). ns, not significant; WT, wild type.

    Article Snippet: EB3 dynamics were captured using a Nikon ECLIPSE Ti2 spinning disk confocal microscope with a 60× objective on a single plane.

    Techniques: Mutagenesis, Staining, Labeling, Imaging